DNAFoil, a novel technology for the rapid detection of food pathogens: Preliminary validation on Salmonella and Listeria monocytogenes

Over the past decades, several tools have been developed for food pathogen detection, including proteomics, metabolomics, immunological, biosensor, and nucleic acid-based approaches. Although these techniques are reliable precise, they time-consuming, technically challenging, costly. Hence, it is necessary to develop rapid which can be performed at household level. DNAFoil mechanism a portable, completely self-administered, on-site DNA test that does not need expensive instru-ments or settings confirm detection in as little 30 min. was used successfully detecting contamination adulteration with pork derivatives (down 0.1%) vegetal components 0.01%), respectively. In this study, initial validation experiments of were investigated detect Listeria monocytogenes Salmonella contamination. To specificity proposed method toward Salmonella, 18 different strains, 6 non-Salmonella bacteria, 2 fungi investigated; also, case monocytogenes, five bacterial two fungi, investigated. The results stated Swiss Decode L. solutions few 1 10 copies per microliter, also showed accuracy our ranges between 92 100%, while precision value ranged 88 100%. terms quality control applicability, reactions could visually detected naked eye using lateral flow strip, in-place during manufacturing more lab tests. cost, usually much cheaper than traditional methods. Therefore, promising universal technology pathogens.